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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">transplantologiya</journal-id><journal-title-group><journal-title xml:lang="ru">Трансплантология</journal-title><trans-title-group xml:lang="en"><trans-title>Transplantologiya. The Russian Journal of Transplantation</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2074-0506</issn><issn pub-type="epub">2542-0909</issn><publisher><publisher-name>IPO Association of Transplantologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.23873/2074-0506-2019-11-2-150-157</article-id><article-id custom-type="elpub" pub-id-type="custom">transplantologiya-433</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КЛИНИЧЕСКИЕ НАБЛЮДЕНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>CASE REPORTS</subject></subj-group></article-categories><title-group><article-title>Случай применения аутологичного тромбофибринового сгустка у пациента с послеожоговой персистирующей эрозией роговицы</article-title><trans-title-group xml:lang="en"><trans-title>A case of autologous thrombofibrin clot use in a patient with post-burn persistent corneal erosion</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8824-3562</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Федосеева</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Fedoseeva</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>врач-офтальмолог, аспирант отдела травматологии и реконструктивной хирургии,</p><p>105062, Москва, Садовая-Черногрязская ул., д. 14/19</p></bio><bio xml:lang="en"><p>Ophthalmologist, Postgraduate of the Department of Trauma and Reconstructive Surgery,</p><p>14/19 Sadovaya-Chernogryazskaya St., Moscow 105062</p></bio><email xlink:type="simple">dr.elfed23@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ченцова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Chentsova</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>проф., д-р мед. наук, руководитель отдела травматологии и реконструктивной хирургии,</p><p>105062, Москва, Садовая-Черногрязская ул., д. 14/19</p></bio><bio xml:lang="en"><p>Prof., Dr. Med. Sci., Head of the Department of Trauma and Reconstructive Surgery,</p><p>14/19 Sadovaya-Chernogryazskaya St., Moscow 105062</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8897-7523</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Боровкова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Borovkova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д-р мед. наук, заведующая научным отделением биотехнологий и трансфузиологии,</p><p>129090, Москва, Большая Сухаревская площадь, д. 3</p></bio><bio xml:lang="en"><p>Dr. Med. Sci., Head of the Scientific Department of Biotechnologies and Transfusiology,</p><p>3 Bolshaya Sukharevskaya Sq., Moscow 129090 </p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2523-6939</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пономарев</surname><given-names>И. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Ponomarev</surname><given-names>I. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. мед. наук, научный сотрудник отделения биотехнологий и трансфузиологии,</p><p>129090, Москва, Большая Сухаревская площадь, д. 3</p></bio><bio xml:lang="en"><p>Cand. Med. Sci., Researcher of the Department of Biotechnologies and Tranfusiology,</p><p>3 Bolshaya Sukharevskaya Sq., Moscow 129090 </p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8888-6090</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Петрова</surname><given-names>А. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Petrova</surname><given-names>A. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник отдела травматологии и реконструктивной хирургии,</p><p>105062, Москва, Садовая-Черногрязская ул., д. 14/19</p></bio><bio xml:lang="en"><p>Senior Researcher of the Department of Trauma and Reconstructive Surgery,</p><p>14/19 Sadovaya-Chernogryazskaya St., Moscow 105062</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Павленко</surname><given-names>Ю. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Pavlenko</surname><given-names>Yu. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>врач-офтальмолог, аспирант отдела пластической хирургии и глазного протезирования,</p><p>105062, Москва, Садовая-Черногрязская ул., д. 14/19</p></bio><bio xml:lang="en"><p>Ophthalmologist, Postgraduate of the Ophthalmic Plastic Surgery and Ocular Prosthetics Department,</p><p>14/19 Sadovaya-Chernogryazskaya St., Moscow 105062</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБУ «Московский НИИ глазных болезней им. Гельмгольца» МЗ РФ</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow Helmholtz’s Research Institute of Eye Diseases</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ГБУЗ «НИИ скорой помощи им. Н.В. Склифосовского ДЗМ»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>N.V. Sklifosovsky Research Institute for Emergency Medicine</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>17</day><month>06</month><year>2019</year></pub-date><volume>11</volume><issue>2</issue><fpage>150</fpage><lpage>157</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Федосеева Е.В., Ченцова Е.В., Боровкова Н.В., Пономарев И.Н., Петрова А.О., Павленко Ю.А., 2019</copyright-statement><copyright-year>2019</copyright-year><copyright-holder xml:lang="ru">Федосеева Е.В., Ченцова Е.В., Боровкова Н.В., Пономарев И.Н., Петрова А.О., Павленко Ю.А.</copyright-holder><copyright-holder xml:lang="en">Fedoseeva E.V., Chentsova E.V., Borovkova N.V., Ponomarev I.N., Petrova A.O., Pavlenko Y.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.jtransplantologiya.ru/jour/article/view/433">https://www.jtransplantologiya.ru/jour/article/view/433</self-uri><abstract><sec><title>Цель</title><p>Цель: оценить на примере клинического наблюдения возможность применения тромбофибринового сгустка и его эффективность в лечении пациентов с послеожоговыми эрозиями роговицы. Материал и методы. Лечение проводили на базе ФГБУ «МНИИ ГБ им. Гельмгольца» МЗ РФ в отделении травматологии и реконструктивной хирургии. Пациенту на основании клинико-инструментальных методов исследования был установлен диагноз: «Постожоговая персистирующая эрозия роговицы с субтотальной конъюнктивизацией, лимбально-клеточная недостаточность правого глаза». Лечение с помощью кератопротекторной терапии было не эффективно на протяжении нескольких месяцев. Для активизации регенерации роговицы эрозия покрыта аутологичным тромбофибриновым сгустком, полученным из стабилизированной крови. После двухэтапного центрифугирования стабилизированной крови больного отбирали плазму с тромбоцитами, помещали ее в пробирку с круглым дном и добавляли активатор хлорид кальция. Сгусток формировался в термостате при 37 °С. Пациенту под местной анестезией сгусток укладывали на поверхность роговицы под амниотическую мембрану. Мембрану фиксировали циркулярным швом по лимбу.</p></sec><sec><title>Результат</title><p>Результат. При наблюдении у пациента отмечали положительную динамику в виде сокращения размеров эрозии на 5-е сут и полной эпителизации роговицы на 14-е сут.</p></sec><sec><title>Заключение</title><p>Заключение. Примененный нами метод, направленный на усиление репаративных и регенераторных процессов в роговице, уменьшение воспалительной реакции, ускорение эпителизации, а также увеличение прозрачности, повышение оптических свойств за счет формирования менее интенсивного помутнения, показал высокую эффективность. </p></sec></abstract><trans-abstract xml:lang="en"><sec><title>The purpose</title><p>The purpose. Using clinical observation, to study the feasibility of applying a coagulant and to assess its efficacy in the treatment of patients with post-burn erosion of the cornea.</p></sec><sec><title>Material and methods</title><p>Material and methods. The treatment was carried out in the Department of Trauma and Reconstructive Surgery, the Helmholtz Moscow Research Institute of Eye Diseases, Russia. Based on clinical test results, the patient, was diagnosed with post-burn persistent erosion of the cornea with sub-total cornea conjunctivization and: limbal stem cell deficiency (LSCD) in the right eye. The keratoprotective therapy conducted for several months turned ineffective. In order to activate the cornea regeneration process, the erosion site was covered with autologous thrombofibrin clot that had been obtained from the stabilized blood. Following a two-stage centrifugation of patient's stabilized blood, the plasma with the platelets were collected into a round-bottomed test-tube; and the chloride calcium activator was added. The clot was formed in the thermostat at a temperature of 37 С. The patient was given a local anesthesia, and the thrombofibrin clot was placed on the cornea surface and covered with an amniotic membrane. The membrane was fixed with a circular stitch along the limbus of the cornea.</p></sec><sec><title>Result</title><p>Result. The patient was followed-up and showed positive changes over time in the form of a diminished erosion area at day 5 and a complete corneal epithelialization at day 14.</p></sec><sec><title>Summary</title><p>Summary. Our methodaimed at enhancing the reparative and regenerative processes in the cornea, reducing swelling, accelerating epithelialization, expanding transparency, and improving the optic characteristics thanks to less intensive opacity formation showed a high efficacy. </p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>роговица</kwd><kwd>ожог</kwd><kwd>персистирующая эрозия роговицы</kwd><kwd>тромбоциты</kwd><kwd>тромбофибриновый сгуток</kwd><kwd>аутологичная плазма</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cornea</kwd><kwd>burn</kwd><kwd>persistent erosion of the cornea</kwd><kwd>platelet count</kwd><kwd>clot</kwd><kwd>autologous plasma</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Dursun D., Kim M.C., Solomon A., Pflugfelder S.C. 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