transplantologiyaТрансплантологияTransplantologiya. The Russian Journal of Transplantation2074-05062542-0909IPO Association of Transplantologists10.23873/2074-0506-2021-13-1-33-40transplantologiya-554Research ArticleАКТУАЛЬНЫЕ ВОПРОСЫ ТРАНСПЛАНТОЛОГИИACTUAL ISSUES OF TRANSPLANTATIONПрогностическое значение уровня сывороточных цитокинов в оценке осложнений после трансплантации печениPredictive value of serum cytokine level in the assessment of complications after liver transplantationhttps://orcid.org/0000-0001-8412-4315МаксимоваА. Ю.MaksimovaA. Yu.

 Арина Юрьевна Максимова, младший научный сотрудник центральной научно-исследовательской лаборатории, отдел общей патологии 

620028, Екатеринбург, ул. Репина, д. 3

 Arina Yu. Maksimova, Junior Researcher of Central Research Laboratory, Department of General Pathology 

3 Repin St., Ekaterinburg 620028

oreshek92@list.ruhttps://orcid.org/0000-0002-4223-3473БессоноваЕ. Н.BessonovaE. N.

 Елена Николаевна Бессонова, д-р мед. наук, заведующая гастроэнтерологическим отделением

620102, Екатеринбург, Волгоградская ул., д. 185

 Elena N. Bessonova, Dr. Sci. (Med.), Head of Gastroenterology Department 

185 Volgogradskaya St., Ekaterinburg 620102

https://orcid.org/0000-0003-0966-9571БазарныйВ. В.BazarnyyV. V.

 Владимир Викторович Базарный, д-р мед. наук, профессор кафедры клинической лабораторной диагностики и бактериологии

620028, Екатеринбург, ул. Репина, д. 3

 Vladimir V. Bazarnyy, Dr. Sci. (Med.), Professor of the Department of Clinical Laboratory Diagnostics and Bacteriology 

3 Repin St., Ekaterinburg 620028

ФГБОУ ВО«Уральский государственный медицинский университет» МЗ РФРоссияUral State Medical UniversityRussian FederationГАУЗ СО «Свердловская областная клиническая больница № 1»РоссияSverdlovsk Regional Clinical Hospital № 1Russian Federation2021240320211313339Copyright © Максимова А.Ю., Бессонова Е.Н., Базарный В.В., 20212021Максимова А.Ю., Бессонова Е.Н., Базарный В.В.Maksimova A.Y., Bessonova E.N., Bazarnyy V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.jtransplantologiya.ru/jour/article/view/554

Введение. Одной из актуальных задач современной трансплантологии является поиск биомаркеров для прогнозирования и ранней диагностики дисфункции трансплантата печени.Цель исследования. Определить биомаркеры дисфункции печеночного трансплантата.Материал и методы. Обследованы 19 реципиентов печеночного трансплантата и 36 условно здоровых доноров крови. Всем обследованным проводили измерение уровня 7 сывороточных цитокинов методом мультипараметрического флуоресцентного анализа с магнитными микросферами (технология xMAP, Luminex 200, USA). Статистический анализ проводили методами непараметрической статистики. Для определения прогностической значимости теста проводили ROC-анализ.Результаты. Мы выявили, что уровень интерлейкина-8 был в 3.6 раз выше у реципиентов с дисфункцией трансплантата по сравнению с теми у кого послеоперационный период протекал без осложнений. Диагностическая чувствительность теста составила 75%, специфичность – 91%, а прогностическая ценность отрицательного результата теста – 84,6.Вывод. Измерение уровня сывороточного интерлейкина-8 является биомаркером раннего прогнозирования развития дисфункции трансплантата печени после трансплантации.

Introduction. One of the urgent tasks in modern transplantology is the search of biomarkers for predicting and early diagnosis of graft dysfunction.Objective. The study objective was to determine the biomarkers of liver graft dysfunction.Material and methods. We have examined 19 recipients who underwent liver transplantation and 36 healthy blood donors. Levels of 7 serum cytokines were measured by multiparametric fluorescence analysis with magnetic microspheres (xMAP technology, Luminex 200, USA). Statistical analysis was carried out by methods of nonparametric statistics. To determine the predictive value of the test, a ROC-analysis was performed.Results and discussion. We found that the interleukin-8 level was 3.6 times higher in recipients with liver graft dysfunction compared to those who had an uneventful postoperative course. The diagnostic sensitivity of the test was 75%, the specificity was 91%, and negative predictive value was 84.6.Conclusion. Serum interleukin-8 measurement provides a biomarker for early predicting a post-transplant liver graft dysfunction development

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The authors declare that there are no conflicts of interest present.