transplantologiyaТрансплантологияTransplantologiya. The Russian Journal of Transplantation2074-05062542-0909IPO Association of Transplantologists10.23873/2074-0506-2022-14-1-79-97transplantologiya-632Research ArticleОБЗОРНЫЕ СТАТЬИ И ЛЕКЦИИREVIEW ARTICLES AND LECTURESДоступные способы повышения регенераторного потенциала пластического материала в неотложной травматологии. Часть 1. Использование аутологичной богатой тромбоцитами плазмы кровиAvailable methods to enhance regenerative potential of plastic materials for bone defects replacement in orthopedics. Part 1. Autologous platelet rich plasmahttps://orcid.org/0000-0001-8616-920XФайнА. М.FaynA. M.

Алексей Максимович Файн, д-р мед. наук, заведующий научным отделением неотложной травматологии опорно-двигательного аппарата; профессор кафедры травматологии, ортопедии и медицины катастроф

129090, Москва, Большая Сухаревская пл., д. 3127473, Москва, Делегатская ул., д. 20, стр. 1

Aleksey M. Fayn, Dr. Sci. (Med.), Head of the Scientific Department of Emergency Traumatology of the Musculoskeletal System; Professor of the Department of Traumatology, Orthopedics, and Disaster Medicine

3 Bolshaya Sukharevskaya Sq., Moscow 12909020 Bldg. 1 Delegatskaya St., Moscow 127473

FainAM@sklif.mos.ruhttps://orcid.org/0000-0003-4581-449XВазаА. Ю.VazaA. Yu.

Александр Юльевич Ваза, канд. мед. наук, ведущий научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Aleksandr Yu. Vaza, Cand. Sci. (Med.), Leading Researcher, the Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

VazaAU@sklif.mos.ruhttps://orcid.org/0000-0001-9932-1653ГнетецкийС. Ф.GnetetskiyS. F.

Сергей Феликсович Гнетецкий, д-р мед. наук, ведущий научный сотрудник отделения неотложной травматологии опорно-двигательного аппарата; доцент кафедры травматологии, ортопедии и медицины катастроф

129090, Москва, Большая Сухаревская пл., д. 3127473, Москва, Делегатская ул., д. 20, стр. 1

Sergey F. Gnetetskiy, Dr. Sci. (Med.), Leading Researcher, the Department of Emergency Traumatology of the Musculoskeletal System; Associate Professor of the Department of Traumatology, Orthopedics, and Disaster Medicine

3 Bolshaya Sukharevskaya Sq., Moscow 12909020 Bldg. 1 Delegatskaya St., Moscow 127473

GnetetskiySF@sklif.mos.ruhttps://orcid.org/0000-0003-3074-453XСкуратовскаяК. И.SkuratovskayaK. I.

Кристина Ивановна Скуратовская, младший научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Kristina I. Skuratovskaya, Junior Researcher, the Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

SkuratovskayaKI@sklif.mos.ruhttps://orcid.org/0000-0002-1183-3644БондаревВ. Б.BondarevV. B.

Василий Бриджевич Бондарев, научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Vasiliy B. Bondarev, Researcher, the Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

BondarevVB@sklif.mos.ruhttps://orcid.org/0000-0002-1509-7082БоголюбскийЮ. А.BogolyubskiyYu. A.

Юрий Андреевич Боголюбский, канд. мед. наук, научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Yuriy A. Bogolyubskiy, Cand. Sci. (Med.), Researcher, the Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

BogoljubskijUA@sklif.mos.ruhttps://orcid.org/0000-0002-2960-8736ТитовР. С.TitovR. S.

Роман Сергеевич Титов, канд. мед. наук, старший научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Roman S. Titov, Cand. Sci. (Med.), Senior Researcher, the Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

TitovRS@sklif.mos.ruhttps://orcid.org/0000-0001-9574-398XСергеевА. Ю.SergeevA. Yu.

Александр Юрьевич Сергеев, научный сотрудник отделения неотложной травматологии

129090, Москва, Большая Сухаревская пл., д. 3

Aleksandr Yu. Sergeev, Researcher, Department of Emergency Traumatology

3 Bolshaya Sukharevskaya Sq., Moscow 129090

SergeevAY@sklif.mos.ruГБУЗ «НИИ скорой помощи им. Н.В. Склифосовского ДЗМ»; ФГБОУ ВО МГМСУ им. А.И. Евдокимова МЗ РФРоссияN.V. Sklifosovsky Research Institute for Emergency Medicine; A.I. Yevdokimov Moscow State University of Medicine and DentistryRussian FederationГБУЗ «НИИ скорой помощи им. Н.В. Склифосовского ДЗМ»РоссияN.V. Sklifosovsky Research Institute for Emergency MedicineRussian Federation2022160320221417997Copyright © Файн А.М., Ваза А.Ю., Гнетецкий С.Ф., Скуратовская К.И., Бондарев В.Б., Боголюбский Ю.А., Титов Р.С., Сергеев А.Ю., 20222022Файн А.М., Ваза А.Ю., Гнетецкий С.Ф., Скуратовская К.И., Бондарев В.Б., Боголюбский Ю.А., Титов Р.С., Сергеев А.Ю.Fayn A.M., Vaza A.Y., Gnetetskiy S.F., Skuratovskaya K.I., Bondarev V.B., Bogolyubskiy Y.A., Titov R.S., Sergeev A.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.jtransplantologiya.ru/jour/article/view/632

Основным методом хирургического лечения пациентов с посттравматическими нарушениями костной регенерации является применение костной пластики. До сих пор оптимальным пластическим материалом является костный аутотрансплантат, для забора которого нужно наносить пациенту дополнительную травму. Альтернативные материалы, которые применяют для пластики, обладают только остеокондуктивным эффектом разной степени выраженности. Для оптимизации свойств пластических материалов, придания им остеостимулирующего эффекта, их можно использовать в комбинации с биологически активными веществами. Источником биологически активных веществ могут служить богатая тромбоцитами плазма, тромбоцитарный лизат и красный костный мозг. Данный обзор литературы включает в себя описание трех основных легкодоступных методов стимуляции остеогенеза. В первой его части рассматриваются механизм действия богатой тромбоцитами плазмы, показания и противопоказания к ее применению, описаны результаты лечения при использовании богатой тромбоцитами плазмы для стимуляции остеогенеза. Богатая тромбоцитами плазма – это продукт собственной крови человека. Изготавливая его путем центрифугирования, получают высокую концентрацию тромбоцитов в небольшом объеме плазмы, которые содержат факторы роста и цитокины, оказывающие непосредственное влияние на процесс регенерации. Для стимуляции остеогенеза проводят местную богатую тромбоцитами плазмы терапию. Вводят аутологичную обогащенную тромбоцитами плазму крови c содержащимися в α-гранулах тромбоцитов факторами роста в аллогенный трансплантат или в зону перелома. Целью данной статьи является обобщение результатов лечения с использованием богатой тромбоцитами плазмы для улучшения регенераторного потенциала в травматологии. Текущие клинические исследования в целом показывают неплохие результаты, особенно при лечении мягких тканей, но высокий уровень клинического подтверждения на данный момент отсутствует.

The main method of surgical treatment of patients with post-traumatic disorders of bone regeneration is the use of bone grafting. Until now, the optimal plastic material has been a bone autograft, which use involves additional trauma to the patient. Alternative materials that are used for grafting have only an osteoconductive effect, of varying effectiveness. To optimize the properties of plastic materials, giving them an osteostimulating effect, they can be used in combination with biologically active substances. A source of biologically active substances can be platelet-rich plasma, platelet lysate and red bone marrow. This literature review includes a description of three main methods to stimulate osteogenesis. The first part examines the mechanism of action of platelet-rich plasma, indications and contraindications for its use, describes the results of treatment when platelet-rich plasma is used to stimulate osteogenesis. Platelet-rich plasma is a product of a human native blood obtained by centrifugation. The output is a high concentration of platelets in a small volume of plasma, which contain growth factors and cytokines that have a direct effect on the regeneration process. Local platelet-rich plasma therapy is performed to stimulate osteogenesis. Autologous platelet-rich plasma with growth factors contained in α-granules of platelets is injected into an allogeneic graft or into a fracture zone. The aim of this article is to summarize the results of treatment using platelet-rich plasma to improve bone regenerative potential in orthopaedics.

регенерация костиложный суставбогатая тромбоцитами плазмафакторы ростакостная пластикаbone regenerationpseudoarthrosisplatelet-rich plasmagrowth factorsbone graftingГосударственное бюджетное финансирование. Работа включена в научно-исследовательский план ГБУЗ «НИИ скорой помощи им. Н.В. Склифосовского ДЗМ».Financed from the State budget. The study has been included in the Research Plan of the N.V. Sklifosovsky Research Institute for Emergency Medicine.ReferencesKausto J, Pentti J, Oksanen T, Virta LJ, Virtanen M, Kivimäki M, et al. Length of sickness absence and sustained return-to-work in mental disorders and musculoskeletal diseases: a cohort study of public sector employees. Scand J Work Environ Health. 2017;43(4):358–366. PMID: 28463382 https://doi.org/10.5271/sjweh.3643Kausto J, Pentti J, Oksanen T, Virta LJ, Virtanen M, Kivimäki M, et al. Length of sickness absence and sustained return-to-work in mental disorders and musculoskeletal diseases: a cohort study of public sector employees. Scand J Work Environ Health. 2017;43(4):358–366. PMID: 28463382 https://doi.org/10.5271/sjweh.3643Рахимов А.М. Стимуляция ауто генным костным мозгом остеорепарации в зоне смоделированного ложного сустава бедренной кости у крыс. Гений ортопедии. 2016;(4):88–94. https://doi.org/10.18019/1028-4427-2016-4-88-94Rakhimov AM. Stimulation of osteoreparation in the zone of modeled femoral pseudarthrosis in rats using autologous bone marrow. Genij Ortopedii. 2016;(4):88–94. (In Russ.). https://doi.org/10.18019/1028-4427-2016-4-88-94Гайко Г.В., Козак Р.А. Факторы риска развития ложных суставов бедренной кости. Политравма. 2012;(4):14–18.Gayko GV, Kozak RA. Risk factors of femur pseudoarthrosis development. Politravma. 2012;(4):14–18. (In Russ.).Zura R, Xiong Z, Einhorn T, Watson JT, Ostrum RF, Prayson MJ, et al. Epidemiology of fracture non union in 18 human bones. JAMA Surg. 2016;151(11):e162775–e162775. PMID: 27603155 https://doi.org/10.1001/jamasurg.2016.2775Zura R, Xiong Z, Einhorn T, Watson JT, Ostrum RF, Prayson MJ, et al. Epidemiology of fracture non union in 18 human bones. JAMA Surg. 2016;151(11):e162775–e162775. PMID: 27603155 https://doi.org/10.1001/jamasurg.2016.2775Xu XP, Huang LL, Hu SL, Han JB, He HL, Xu JY, et al. Genetic modification of mesenchymal stem cells overexpressing angiotensin II type 2 receptor increases cell migration to injured lung in LPS-induced acute lung injury mice. Stem Сells Transl Med. 2018;7(10):721–730. PMID: 30133167 https://doi.org/10.1002/sctm.17-0279Xu XP, Huang LL, Hu SL, Han JB, He HL, Xu JY, et al. Genetic modification of mesenchymal stem cells overexpressing angiotensin II type 2 receptor increases cell migration to injured lung in LPS-induced acute lung injury mice. Stem Сells Transl Med. 2018;7(10):721–730. PMID: 30133167 https://doi.org/10.1002/sctm.17-0279Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res. 2014;9(1):18. PMID: 24628910 https://doi.org/10.1186/1749-799X-9-18Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res. 2014;9(1):18. PMID: 24628910 https://doi.org/10.1186/1749-799X-9-18Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: current concepts and future directions. BMC Med. 2011;9:66. PMID: 21627784 https://doi.org/10.1186/1741-7015-9-66Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: current concepts and future directions. BMC Med. 2011;9:66. PMID: 21627784 https://doi.org/10.1186/1741-7015-9-66Кирилова И.А., Садовой М.А., Подорожная В.Т. Сравнительная характеристика материалов для костной пластики: состав и свойства. Хирургия позвоночника. 2012;3:72–83.Kirilova IA, Sadovoy MA, Podorozhnaya VT. Comparative characteristics of materials for bone grafting: composition and properties. Hirurgiâ pozvonočnika (Spine Surgery). 2012;(3):72–83. (In Russ.). https://doi.org/10.14531/ss2012.3.72-83Панкратов А.С., Лекишвили М.В., Копецкий И.С. (ред.) Костная пластика в стоматологии и челюстно-лицевой хирургии: руководство для врачей. Москва: Бином; 2011.Pankratov AS, Lekishvili MV, Kopetskiy IS. (eds.) Kostnaya plastika v stomatologii i chelyustno-litsevoy khirurgii: rukovodstvo dlya vrachey. Moscow: Binom Publ.; 2011. (In Russ.).Hall MP, Band PA, Meislin RJ, Jazrawi LM, Cardone DA. Platelet-rich plasma: current concepts and application in sports medicine. J Am Acad Orthop Surg. 2009;17(10):602–608. PMID: 19794217 https://doi.org/10.5435/00124635-200910000-00002Hall MP, Band PA, Meislin RJ, Jazrawi LM, Cardone DA. Platelet-rich plasma: current concepts and application in sports medicine. J Am Acad Orthop Surg. 2009;17(10):602–608. PMID: 19794217 https://doi.org/10.5435/00124635-200910000-00002Lubowitz JH, Poehling GG. Shoulder, hip, knee, and PRP. Arthroscopy. 2010; 26(2):141–142. PMID: 20141973 https://doi.org/10.1016/j.arthro.2009.12.005Lubowitz JH, Poehling GG. Shoulder, hip, knee, and PRP. Arthroscopy. 2010; 26(2):141–142. PMID: 20141973 https://doi.org/10.1016/j.arthro.2009.12.005Martinez S. Practical guidelines for using PRP in the orthopaedic office. Available at: http://davidhalseymd.com/wp-content/uploads/2018/06/Practical-guidelines-for-using-PRP-in-the-orthopaedic-office.pdf [Accessed December 11, 2021].Martinez S. Practical guidelines for using PRP in the orthopaedic office. Available at: http://davidhalseymd.com/wp-content/uploads/2018/06/Practical-guidelines-for-using-PRP-in-the-orthopaedic-office.pdf [Accessed December 11, 2021].Nurden AT. Platelets, inflammation and tissue regeneration. Thromb Haemost. 2011;105(Suppl 1): S13–33. PMID: 21479340 https://doi.org/10.1160/THS10-11-0720Nurden AT. Platelets, inflammation and tissue regeneration. Thromb Haemost. 2011;105(Suppl 1): S13–33. PMID: 21479340 https://doi.org/10.1160/THS10-11-0720Shih DT, Burnouf T. Preparation, quality criteria, and properties of human blood platelet lysate supplements for ex vivo stem cell expansion. N Biotech. 2015;32(1):199–211. PMID: 24929129 https://doi.org/10.1016/j.nbt.2014.06.001Shih DT, Burnouf T. Preparation, quality criteria, and properties of human blood platelet lysate supplements for ex vivo stem cell expansion. N Biotech. 2015;32(1):199–211. PMID: 24929129 https://doi.org/10.1016/j.nbt.2014.06.001Ахмеров Р.Р., Зарудий Р.Ф., Бочкова О.И., Рычкова Н.Н. Плазмолифтинг (Plasmolifting) – лечение возрастной атрофии кожи богатой тромбоцитами аутоплазмой. Эстетическая медицина. 2011;Х(2):3–9.Akhmerov RR, Zarudiy RF, Bochkova OI, Rychkova NN. Plazmolifting («PlasmoliftingТМ») – lechenie vozrastnoy atrofii kozhi bogatoi trombotsitami autoplazmoi. Esteticheskaya meditsina. 2011;Х(2):181–187. (In Russ.).Malhotra A, Pelletier M, Oliver R, Christou C, Walsh WR. Platelet-rich plasma and bone defect healing. Tissue Eng Part A. 2014;20(19–20):2614-2633. PMID: 24666439 https://doi.org/10.1089/ten.TEA.2013.0737Malhotra A, Pelletier M, Oliver R, Christou C, Walsh WR. Platelet-rich plasma and bone defect healing. Tissue Eng Part A. 2014;20(19–20):2614-2633. PMID: 24666439 https://doi.org/10.1089/ten.TEA.2013.0737Araki J, Jona M, Eto H, Aoi N, Kato H, Suga H, et al. Optimized preparation method of platelet-concentrated plasma and noncoagulating plateletderived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng Part C Methods. 2012;18(3):176–185. PMID: 21951067 https://doi.org/10.1089/ten.TEC.2011.0308Araki J, Jona M, Eto H, Aoi N, Kato H, Suga H, et al. Optimized preparation method of platelet-concentrated plasma and noncoagulating plateletderived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng Part C Methods. 2012;18(3):176–185. PMID: 21951067 https://doi.org/10.1089/ten.TEC.2011.0308Foster TE, Puskas BL, Mandel baum BR, Gerhardt MB, Rodeo SA. Platelet-rich plasma: from basic sci ence to clinical applications. Am J Sports Med. 2009;37(11):2259-2272. PMID: 19875361 https://doi.org/10.1177/0363546509349921Foster TE, Puskas BL, Mandel baum BR, Gerhardt MB, Rodeo SA. Platelet-rich plasma: from basic sci ence to clinical applications. Am J Sports Med. 2009;37(11):2259-2272. PMID: 19875361 https://doi.org/10.1177/0363546509349921Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91(1):4–15. PMID: 14691563 https://doi.org/10.1160/TH03-07-0440Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91(1):4–15. PMID: 14691563 https://doi.org/10.1160/TH03-07-0440Хватов В.Б., Макаров М.С., Боровкова Н.В. Морфологическая оценка адгезивной активности тромбоцитов с помощью витального окрашивания. Клиническая лабораторная диагностика. 2013;(7):58–61.Khvatov VB, Makarov MS, Borovkova NV. The morphologic evaluation of adhesive activity of human thrombocytes using vital dye. Klinicheskaya laboratornaya diagnostika. 2013;(7):58–61. (In Russ.)Ваза А.Ю., Макаров М.С., Сластинин В.В., Боровкова Н.В., Клюквин И.Ю., Похитонов Д.Ю. и др. Эффективность комбинации аллогенной богатой тромбоцитами плазмы с коллагеном при лечении дефектов бедренной кости у крыс. Трансплантология. 2016;(2):36–44.Vaza AYu, Makarov MS, Slas tinin VV, Borovkova NV, Klyukvin IYu, Pokhitonov DYu, et al. Efficiency of allogenic platelet-rich plasmsa, combined with collagen, in rat's humerus injury healing. Transplantologiya. The Russian Journal of Transplantation. 2016;(2):36–44. (In Russ.).Peltier LF. Fractures: a history and iconography of their treatment. San Francisco: Norman Publ; 1990.Peltier LF. Fractures: a history and iconography of their treatment. San Francisco: Norman Publ; 1990.Ачкасов Е.Е., Безуглов Э.Н., Ульянов А.А., Куршев В.Е., Репетюк А.Д., Егорова О.Н. Применение аутоплаз мы, обогащенной тромбоцитами, в клинической практике. Биомедицина. 2013;4:46–59.Achkasov EE, Bezuglov EN, Ulyanov AA, Kurshev VE, Repetyuk AD, Egorova ON. Application platelet-rich plasma in clinical practice. Biomedicine. 2013;4:46–59. (In Russ.).Urist MR, Strates BS. Bone morphogenetic protein. J Dent Res. 1971;50(6):1392–1406. PMID: 4943222 https://doi.org/10.1177/00220345710500060601Urist MR, Strates BS. Bone morphogenetic protein. J Dent Res. 1971;50(6):1392–1406. PMID: 4943222 https://doi.org/10.1177/00220345710500060601Gimbrone MA, Aster RH, Cotran RS, Corkery J, Jandl JH, Folkman J. Preservation of vascular integrity in organs perfused in vitro with a platelet rich medium. Nature. 1969;222:33–36. PMID: 5775827 https://doi.org/10.1038/222033a0Gimbrone MA, Aster RH, Cotran RS, Corkery J, Jandl JH, Folkman J. Preservation of vascular integrity in organs perfused in vitro with a platelet rich medium. Nature. 1969;222:33–36. PMID: 5775827 https://doi.org/10.1038/222033a0Marx RE. Radiation injury to tis sue. In: Kindwall ER, Whelan HT. (eds.) Hyperbaric Medicine Practice. 3 rd ed. Best Publishing; 2008. p. 853–903.Marx RE. Radiation injury to tis sue. In: Kindwall ER, Whelan HT. (eds.) Hyperbaric Medicine Practice. 3 rd ed. Best Publishing; 2008. p. 853–903.Ахмеров Р.Р. (ред.) Регенеративная медицина на основе аутологичной плазмы. Технология Плазмолифтинг («PlasmoliftingТМ»). Москва: Литтерра; 2012.Akhmerov RR. (eds.) Regenerativnaya medicina na osnove autologichnoy plasmy. Tekhnologiya Plazmolifting («PlasmoliftingТМ»). Moscow: Litterra Publ.; 2012. (In Russ.).Фриденштейн А.Я., Лалыкина К.С. Индукция костной ткани и остеогенные клетки-предшественники. Москва: Медицина; 1973.Fridenshteyn AYa, Lalykina KS. Induktsiya kostnoy tkany I osteogenny kletki-predshestvenniki. Мoscow: Medicina Publ.; 1973. (In Russ.).Urist MR. Bone: formation by autoinduction. Science. 1975;150(3698): 893–899. PMID: 5319761 https://doi.org/10.1126/science.150.3698.893Urist MR. Bone: formation by autoinduction. Science. 1975;150(3698): 893–899. PMID: 5319761 https://doi.org/10.1126/science.150.3698.893Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 1999;14(4):529–535. PMID: 10453668Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 1999;14(4):529–535. PMID: 10453668Oryan A, Parizi AM, Shafiei-Sarvestani Z, Bigham AS. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model: radiological, macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank. 2012;13(4):639–651. PMID: 22180011 https://doi.org/10.1007/s10561-011-9285-xOryan A, Parizi AM, Shafiei-Sarvestani Z, Bigham AS. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model: radiological, macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank. 2012;13(4):639–651. PMID: 22180011 https://doi.org/10.1007/s10561-011-9285-xAlsousou J, Thompson M, Hulley P, Noble A, Willett K. The biology of platelet-rich plasma and its application in trauma and orthopedic surgery: a review of the literature. J Bone Joint Surg B. 2009;91(8):987–996. PMID: 19651823 https://doi.org/10.1302/0301-620XAlsousou J, Thompson M, Hulley P, Noble A, Willett K. The biology of platelet-rich plasma and its application in trauma and orthopedic surgery: a review of the literature. J Bone Joint Surg B. 2009;91(8):987–996. PMID: 19651823 https://doi.org/10.1302/0301-620XKao HK, Chen B, Murphy GF, Li Q, Orgill DP, Guo L. Peripheral blood fibrocytes: enhancement of wound healing by cell proliferation, re-epithelialization, contraction, and angiogenesis. Ann Surg. 2011;254(6):1066–1074. PMID: 21832942 https://doi.org/10.1097/SLA.0b013e3182251559Kao HK, Chen B, Murphy GF, Li Q, Orgill DP, Guo L. Peripheral blood fibrocytes: enhancement of wound healing by cell proliferation, re-epithelialization, contraction, and angiogenesis. Ann Surg. 2011;254(6):1066–1074. PMID: 21832942 https://doi.org/10.1097/SLA.0b013e3182251559Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstruct Surg. 2006;118(6):147e–159e. PMID: 17051095 https://doi.org/10.1097/01.prs.0000239606.92676.cfEppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstruct Surg. 2006;118(6):147e–159e. PMID: 17051095 https://doi.org/10.1097/01.prs.0000239606.92676.cfHsiao ST, Asgari A, Lokmic Z, Sinclair R, Dusting GJ, Lim SY, et al. Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue. Stem Cells Dev. 2012;21(12):2189–2203. PMID: 22188562 https://doi.org/10.1089/scd.2011.0674Hsiao ST, Asgari A, Lokmic Z, Sinclair R, Dusting GJ, Lim SY, et al. Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue. Stem Cells Dev. 2012;21(12):2189–2203. PMID: 22188562 https://doi.org/10.1089/scd.2011.0674Panseri S, Russo A, Cunha C, Bondi A, Di Martino A, Patella S, et al. Osteochondral tissue engineering approaches for articular cartilage and subchondral bone regeneration. Knee Surg Sports Traumatol Arthrosc. 2012;20(6):1182-1191. PMID: 21910001 https://doi.org/10.1007/s00167-011-1655-1Panseri S, Russo A, Cunha C, Bondi A, Di Martino A, Patella S, et al. Osteochondral tissue engineering approaches for articular cartilage and subchondral bone regeneration. Knee Surg Sports Traumatol Arthrosc. 2012;20(6):1182-1191. PMID: 21910001 https://doi.org/10.1007/s00167-011-1655-1Mohan S, Baylink DJ. IGF-bin ding proteins are multifunctional and act via IGF-dependent and -inde pendent mecha nisms. J Endocrinol. 2002;175(1):19–31. PMID: 12379487 https://doi.org/10.1677/joe.0.1750019Mohan S, Baylink DJ. IGF-bin ding proteins are multifunctional and act via IGF-dependent and -inde pendent mecha nisms. J Endocrinol. 2002;175(1):19–31. PMID: 12379487 https://doi.org/10.1677/joe.0.1750019Govoni KE, Baylink DJ, Mohan S. Themulti-functional role of insulinlike growth factor binding proteins in bone. Pediatr Nephrol. 2005;20(3):261–268. PMID: 15549410 https://doi.org/10.1007/s0467-004-1658-yGovoni KE, Baylink DJ, Mohan S. Themulti-functional role of insulinlike growth factor binding proteins in bone. Pediatr Nephrol. 2005;20(3):261–268. PMID: 15549410 https://doi.org/10.1007/s0467-004-1658-yBehr B, Tang C, Germann G, Longaker MT, Quarto N. Locally applied vascular endothelial growth factor A increases the osteogenic healing capacity of human adipose-derived stem cells by promoting osteogenic and endothelial differentiation. Stem Cells. 2011;29(2):286–296. PMID: 21732486 https://doi.org/10.1002/stem.581Behr B, Tang C, Germann G, Longaker MT, Quarto N. Locally applied vascular endothelial growth factor A increases the osteogenic healing capacity of human adipose-derived stem cells by promoting osteogenic and endothelial differentiation. Stem Cells. 2011;29(2):286–296. PMID: 21732486 https://doi.org/10.1002/stem.581Holstein JH, Becker SC, Fiedler M, Garcia P, Histing T, Klein M, et al. Intravital microscopic studies of angiogenesis during bone defect healing in mice calvaria. Injury. 2011;42(8):765–771. PMID: 21156316 https://doi.org/10.1016/j.injury.11.020Holstein JH, Becker SC, Fiedler M, Garcia P, Histing T, Klein M, et al. Intravital microscopic studies of angiogenesis during bone defect healing in mice calvaria. Injury. 2011;42(8):765–771. PMID: 21156316 https://doi.org/10.1016/j.injury.11.020Karp M, Tanaka TS, Zohar R, Sodek J, Shoichet MS, Davies JE, et al. Thrombin mediated migration of osteogenic cells. Bone. 2005;37(3):337–348. PMID: 15964256 https://doi.org/10.1016/j.bone.2005.04.022Karp M, Tanaka TS, Zohar R, Sodek J, Shoichet MS, Davies JE, et al. Thrombin mediated migration of osteogenic cells. Bone. 2005;37(3):337–348. PMID: 15964256 https://doi.org/10.1016/j.bone.2005.04.022Annabi B, Thibeault S, Lee YT, Bousquet-Gagnon N, Eliopoulos N, Barrette S, et al. Matrix metalloproteinase regulation of sphingosine-1-phosphateinduced angiogenic properties of bone marrow stromal cells. Exp Hematol. 2003;31(7):640–649. PMID: 12842709 https://doi.org/10.1016/s0301-472x(03)00090-0Annabi B, Thibeault S, Lee YT, Bousquet-Gagnon N, Eliopoulos N, Barrette S, et al. Matrix metalloproteinase regulation of sphingosine-1-phosphateinduced angiogenic properties of bone marrow stromal cells. Exp Hematol. 2003;31(7):640–649. PMID: 12842709 https://doi.org/10.1016/s0301-472x(03)00090-0Ball SG, Shuttleworth CA, Kiel ty CM. Mesenchymal stem cells and neovascularization: role of plateletderived growth factor receptors. J Cell Mol Med. 2007;11(5):1012–1030. PMID: 17979880 https://doi.org/10.1111/j1582-4934.2007.00120.xBall SG, Shuttleworth CA, Kiel ty CM. Mesenchymal stem cells and neovascularization: role of plateletderived growth factor receptors. J Cell Mol Med. 2007;11(5):1012–1030. PMID: 17979880 https://doi.org/10.1111/j1582-4934.2007.00120.xKamoda H, Yamashita M, Ishikawa T, Miyagi M, Arai G, Suzuki M, et al. Platelet-rich plasma combined with hydroxyapatite for lumbar interbody fusion promoted bone formation and decreased an inflammatory pain neuropeptide in rats. Spine (Phila Pa 1976). 2012;37(20):17271733. PMID: 22433505 https://doi.org/10.1097/BRS.0b013e31825567b7Kamoda H, Yamashita M, Ishikawa T, Miyagi M, Arai G, Suzuki M, et al. Platelet-rich plasma combined with hydroxyapatite for lumbar interbody fusion promoted bone formation and decreased an inflammatory pain neuropeptide in rats. Spine (Phila Pa 1976). 2012;37(20):17271733. PMID: 22433505 https://doi.org/10.1097/BRS.0b013e31825567b7Mishra A, Woodall J, Vieira A. Treatment of tendon and muscle using platelet-rich plasma. Clin Sports Med. 2009;28(1):113–125. PMID: 19064169 https://doi.org/10.1016/j.csm.2008.08.007Mishra A, Woodall J, Vieira A. Treatment of tendon and muscle using platelet-rich plasma. Clin Sports Med. 2009;28(1):113–125. PMID: 19064169 https://doi.org/10.1016/j.csm.2008.08.007David JP, Schett G. TNF and bone. Curr Dir Autoimmun. 2010;11:135–144. PMID: 20173392 https://doi.org/10.1159/000289202David JP, Schett G. TNF and bone. Curr Dir Autoimmun. 2010;11:135–144. PMID: 20173392 https://doi.org/10.1159/000289202Lyras DN, Kazakos K, Verettas D, Botaitis S, Agrogiannis G, Kokka A, et al. The effect of platelet-rich plasma gel in the early phase of patellar tendon healing. Arch Orthop Trauma Surg. 2009;129(11):1577–1582. PMID: 19621231 https://doi.org/10.1007/s00402-009-0935-4Lyras DN, Kazakos K, Verettas D, Botaitis S, Agrogiannis G, Kokka A, et al. The effect of platelet-rich plasma gel in the early phase of patellar tendon healing. Arch Orthop Trauma Surg. 2009;129(11):1577–1582. PMID: 19621231 https://doi.org/10.1007/s00402-009-0935-4Cachacёo AS, Carvalho T, Santos AC, Igreja C, Fragoso R, Osório C, et al. TNF-α regulates the effects of irradiation in the mouse bone marrow microenvironment. PLoS ONE. 2010;5(2):e8980. PMID: 20126546 https://doi.org/10.1371/journal.pone.0008980Cachacёo AS, Carvalho T, Santos AC, Igreja C, Fragoso R, Osório C, et al. TNF-α regulates the effects of irradiation in the mouse bone marrow microenvironment. PLoS ONE. 2010;5(2):e8980. PMID: 20126546 https://doi.org/10.1371/journal.pone.0008980Taylor DW, Petrera M, Hendry M, Theodoropoulos JS. A systematic review of the use of platelet-rich plasma in sports medicine as a new treatment for tendon and ligament injuries. Clin J Sport Med. 2011;21(4):344–352. PMID: 21562414 https://doi.org/10.1097/JSM.0b013e31821d0f65Taylor DW, Petrera M, Hendry M, Theodoropoulos JS. A systematic review of the use of platelet-rich plasma in sports medicine as a new treatment for tendon and ligament injuries. Clin J Sport Med. 2011;21(4):344–352. PMID: 21562414 https://doi.org/10.1097/JSM.0b013e31821d0f65Hakimi M, Jungbluth P, Sager M, Betsch M, Herten M, Becker J, et al. Combined use of platelet-rich plasma and autologous bone grafts in the treatment of long bone defects in mini-pigs. Injury. 2010;41(7):717–723. PMID: 20097341 https://doi.org/10.1016/j.injury.2009.12.005Hakimi M, Jungbluth P, Sager M, Betsch M, Herten M, Becker J, et al. Combined use of platelet-rich plasma and autologous bone grafts in the treatment of long bone defects in mini-pigs. Injury. 2010;41(7):717–723. PMID: 20097341 https://doi.org/10.1016/j.injury.2009.12.005Yamada Y, Ueda M, Naiki T, Takahashi M, Hata KI, Nagasaka T. Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: tissue engineered bone regeneration. Tissue Engineering. 2004;10(5–6):955–964. PMID: 15265313 https://doi.org/10.1089/1076327041348284Yamada Y, Ueda M, Naiki T, Takahashi M, Hata KI, Nagasaka T. Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: tissue engineered bone regeneration. Tissue Engineering. 2004;10(5–6):955–964. PMID: 15265313 https://doi.org/10.1089/1076327041348284Han B, Woodell-May J, Pontici ello M, Yang Z, Nimni M. The effect of thrombin activation of platelet-rich plasma on demineralized bone matrix osteoinductivity. J BoneJoint Surg A. 2009;91(6):1459–1470. PMID: 19487525 https://doi.org/10.2106/JBJS.H.00246Han B, Woodell-May J, Pontici ello M, Yang Z, Nimni M. The effect of thrombin activation of platelet-rich plasma on demineralized bone matrix osteoinductivity. J BoneJoint Surg A. 2009;91(6):1459–1470. PMID: 19487525 https://doi.org/10.2106/JBJS.H.00246Giovanini AF, Deliberador TM, Gonzaga CC, de Oliveira Filho MA, Göhringer I, Kuczera J, et al. Platelet-rich plasma diminishes calvarial bone repair associated with alterations in collagen matrix composition and elevated CD34+ cell prevalence. Bone. 2010;46(6):15971603. PMID: 20206725 https://doi.org/10.1016/j.bone.2010.02.026Giovanini AF, Deliberador TM, Gonzaga CC, de Oliveira Filho MA, Göhringer I, Kuczera J, et al. Platelet-rich plasma diminishes calvarial bone repair associated with alterations in collagen matrix composition and elevated CD34+ cell prevalence. Bone. 2010;46(6):15971603. PMID: 20206725 https://doi.org/10.1016/j.bone.2010.02.026Lam FW, Vijayan KV, Rumbaut RE. Platelets and their interactions with other immune cells. Compr Physiol. 2015;5(3):1265–1280. PMID: 26140718 https://doi.org/10.1002/cphy.c140074Lam FW, Vijayan KV, Rumbaut RE. Platelets and their interactions with other immune cells. Compr Physiol. 2015;5(3):1265–1280. PMID: 26140718 https://doi.org/10.1002/cphy.c140074Mlynarek RA, Kuhn AW, Bedi A. Platelet-rich plasma (PRP) in orthopedic sports medicine. Am J Orthop (Belle Mead NJ). 2016;45(5):290–326. PMID: 27552452Mlynarek RA, Kuhn AW, Bedi A. Platelet-rich plasma (PRP) in orthopedic sports medicine. Am J Orthop (Belle Mead NJ). 2016;45(5):290–326. PMID: 27552452Flynn JM. Fracture repair and bone grafting. In: OKU 10: Orthopaedic knowledge update. Rosemont, IL: American Academy of Orthopaedic Surgeons; 2011. p. 11–21.Flynn JM. Fracture repair and bone grafting. In: OKU 10: Orthopaedic knowledge update. Rosemont, IL: American Academy of Orthopaedic Surgeons; 2011. p. 11–21.Calori GM, Tagliabue L, Gala L, D'Imporzano M, Peretti G, Albisetti W. Application of rhBMP-7 and plateletrich plasma in the treatment of long bone non-unions. Injury. 2008;39(12):1391-1402. PMID: 19027898 https://doi.org/10.1016/j.injury.2008.08.011Calori GM, Tagliabue L, Gala L, D'Imporzano M, Peretti G, Albisetti W. Application of rhBMP-7 and plateletrich plasma in the treatment of long bone non-unions. Injury. 2008;39(12):1391-1402. PMID: 19027898 https://doi.org/10.1016/j.injury.2008.08.011Gołos J, Walinski T, Piekarczyk P, Kwiatkowski K. Results of the use of platelet rich plasma in the treatment of delayed union of long bones. Ortop Traumatol Rehabil. 2013;16(4):397–406. PMID: 25404629 https://doi.org/10.5604/15093492.1119617Gołos J, Walinski T, Piekarczyk P, Kwiatkowski K. Results of the use of platelet rich plasma in the treatment of delayed union of long bones. Ortop Traumatol Rehabil. 2013;16(4):397–406. PMID: 25404629 https://doi.org/10.5604/15093492.1119617Malhotra R, Kumar V, Garg B, Singh R, Jain V, Coshic P. Chatterjee K. Role of autologous platelet-rich plasma in treatment of long-bone non-unions: a prospective study. Musculoskelet Surg. 2015;99(3):243–248. PMID: 26193983 https://doi.org/10.1007/s12306-015-0378-8Malhotra R, Kumar V, Garg B, Singh R, Jain V, Coshic P. Chatterjee K. Role of autologous platelet-rich plasma in treatment of long-bone non-unions: a prospective study. Musculoskelet Surg. 2015;99(3):243–248. PMID: 26193983 https://doi.org/10.1007/s12306-015-0378-8Oryan A, Alidadi S, Moshiri A. Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. 2016;16(2):213–232. PMID: 26561282 https://doi.org/10.1517/14712598.2016.1118458Oryan A, Alidadi S, Moshiri A. Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. 2016;16(2):213–232. PMID: 26561282 https://doi.org/10.1517/14712598.2016.1118458Roffi A, Di Matteo B, Krishnakumar GS, Kon E, Filardo G. Platelet-rich plasma for the treatment of bone defects: from pre-clinical rational to evidence in the clinical practice. A systematic review. Int Orthop. 2016;41(2):221–237. PMID: 27888295 https://doi.org/10.1007/s00264-016-3342-9Roffi A, Di Matteo B, Krishnakumar GS, Kon E, Filardo G. Platelet-rich plasma for the treatment of bone defects: from pre-clinical rational to evidence in the clinical practice. A systematic review. Int Orthop. 2016;41(2):221–237. PMID: 27888295 https://doi.org/10.1007/s00264-016-3342-9Макаров М.С., Кобзева Е.Н., Высочин И.В., Боровкова Н.В., Хватов В.Б. Морфофункциональный анализ тромбоцитов человека с помощью витального окрашивания. Бюллетень экспериментальной биологии и медицины. 2013;156(9):388–391.Makarov MS, Kobzeva EN, Vysochin IV, Borovkova NV, Khvatov VB. Morfofunktsional'nyy analiz trombotsitov cheloveka s pomoshch'yu vital'nogo okrashivaniya. Bulletin of Experimental Biology and Medicine. 2013;156(9):388–391. (In Russ.).Guzel Y, Karalezli N, Bilge O, Kacira BK, Esen H, Karadag H, et al. The biomechanical and histological effects of platelet-rich plasma on fracture healing. Knee Surg Sports Traumatol Arthrosc. 2015;23(5):1378–1383. PMID: 24170188 https://doi.org/10.1007/s00167-013-2734-2Guzel Y, Karalezli N, Bilge O, Kacira BK, Esen H, Karadag H, et al. The biomechanical and histological effects of platelet-rich plasma on fracture healing. Knee Surg Sports Traumatol Arthrosc. 2015;23(5):1378–1383. PMID: 24170188 https://doi.org/10.1007/s00167-013-2734-2Блаженко А.Н., Родин И.А., Понкина О.Н., Муханов М.Л., Самойлова А.С., Веревкин А.А. и др. Влияние А-PRPтерапии на репаративную регенерацию костной ткани при свежих переломах костей конечностей. Инновационная медицина Кубани. 2019;3(15):32–38. https://doi.org/10.35401/2500-0268-2019-15-3-32-38Blazhenko AN, Rodin IA, Ponkina ON, Mukhanov ML, Samoilova AS, Verevkin AA, et al. The effect of A-PRPtherapy on reparative regeneration of bone tissue with acute bone fractures of the limbs. Innovative Medicine of Kuban. 2019;3(15):32–38. (In Russ.). https://doi.org/10.35401/2500-0268-2019-15-3-32-38Schneppendahl J, Jungbluth P, Lögters T, Sager M, Wild M, Hakimi M, et al. Treatment of a diaphyseal long-bone defect with autologous bone grafts and platelet-rich plasma in a rabbit model. Vet Comp Orthop Traumatol. 2015;28(3):164–171. PMID: 25804295 https://doi.org/10.3415/VCOT-14-05-0079Schneppendahl J, Jungbluth P, Lögters T, Sager M, Wild M, Hakimi M, et al. Treatment of a diaphyseal long-bone defect with autologous bone grafts and platelet-rich plasma in a rabbit model. Vet Comp Orthop Traumatol. 2015;28(3):164–171. PMID: 25804295 https://doi.org/10.3415/VCOT-14-05-0079Henschler R, Gabriel C, Schall moser K, Burnouf T, Koh MB. Human platelet lysate current standards and future developments. Transfusion. 2019;59(4):1407–1413. PMID: 30741431 https://doi.org/10.1111/trf.15174Henschler R, Gabriel C, Schall moser K, Burnouf T, Koh MB. Human platelet lysate current standards and future developments. Transfusion. 2019;59(4):1407–1413. PMID: 30741431 https://doi.org/10.1111/trf.15174Ranly D, Lohmann C, Boyan DB, Schwartz Z. Platelet-rich plasma inhibits demineralized bone matrix-induced bone formation in nude mice. J Bone Joint Surg Am. 2007;89(1):139–147. PMID: 17200321 https://doi.org/10.2106/JBJS.F.00388Ranly D, Lohmann C, Boyan DB, Schwartz Z. Platelet-rich plasma inhibits demineralized bone matrix-induced bone formation in nude mice. J Bone Joint Surg Am. 2007;89(1):139–147. PMID: 17200321 https://doi.org/10.2106/JBJS.F.00388Макаров М.С. Перспективы использования тромбоцитов человека в лечении дефектов костной ткани. Наука и мир. 2019;2(2):62–66.Makarov MS. Prospects for the use of human platelets in the treatment of bone defects. Science and world. 2013;2(2):62-66. (In Russ.).Esposito M, Grusovin MG, Rees J, Karasoulos D, Felice P, Alissa R. Interventions for replacing missing teeth: augmentation procedures of the maxillary sinus. Cochrane Database Syst Rev. 2010;(3):CD008397. PMID: 24825543 https://doi.org/10.1002/14651858.CD008397.pub2Esposito M, Grusovin MG, Rees J, Karasoulos D, Felice P, Alissa R. Interventions for replacing missing teeth: augmentation procedures of the maxillary sinus. Cochrane Database Syst Rev. 2010;(3):CD008397. PMID: 24825543 https://doi.org/10.1002/14651858.CD008397.pub2Griffin XL, Smith CM, Costa ML. The clinical use of platelet-rich plasma in the promotion of bone healing: a systematic review. Injury. 2009;40(2):158–162. PMID: 19084836 https://doi.org/10.1016/j.injury.2008.06.025Griffin XL, Smith CM, Costa ML. The clinical use of platelet-rich plasma in the promotion of bone healing: a systematic review. Injury. 2009;40(2):158–162. PMID: 19084836 https://doi.org/10.1016/j.injury.2008.06.025

The authors declare that there are no conflicts of interest present.