transplantologiyaТрансплантологияTransplantologiya. The Russian Journal of Transplantation2074-05062542-0909IPO Association of Transplantologiststransplantologiya-98Research ArticleАКТУАЛЬНЫЕ ВОПРОСЫ ТРАНСПЛАНТОЛОГИИACTUAL ISSUES OF TRANSPLANTATIONВлияние пероксиредоксина VI на сохранение тонкой кишки крысы при ишемии/реперфузииThe effects of Peroxiredoxin VI on the preservation of the small intestine in rats after ischemia/reperfusion damageГордееваА. Е.GordeevaA. E.ШараповМ. Г.SharapovV. G.НовоселовВ. И.NovoselovV. I.ФесенкоЕ. Е.FesenkoE. E.ТемновА. А.TemnovA. A.aa-temnov@yandex.ruХубутияМ. Ш.KhubutiyaM. Sh.ФГБУН «Институт биофизики клетки Российской академии наук», ПущиноРоссияInstitute of сell biophysics Russian Academy of Sciences, PushchinoRussian FederationГБУЗ «НИИ скорой помощи им. Н.В. Склифосовского ДЗМ», МоскваРоссияN.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, MoscowRussian Federation201405022016042127Copyright © Гордеева А.Е., Шарапов М.Г., Новоселов В.И., Фесенко Е.Е., Темнов А.А., Хубутия М.Ш., 20162016Гордеева А.Е., Шарапов М.Г., Новоселов В.И., Фесенко Е.Е., Темнов А.А., Хубутия М.Ш.Gordeeva A.E., Sharapov V.G., Novoselov V.I., Fesenko E.E., Temnov A.A., Khubutiya M.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.jtransplantologiya.ru/jour/article/view/98

Высокая потребность кишечника в кислороде делает его весьма чувствительным к ишемически-реперфузионному поражению (И/Р). Целью данной работы является исследование влияния пероксиредоксина VI (Prx VI) на сохранение тонкой кишки при И/Р-повреждении в модели странгуляционной тонкокишечной непроходимости. Моделирование тонкокишечной странгуляционной непроходимости было выполнено путем окклюзии дистальной петли подвздошной кишки и сосудов брыжейки в течение 60 минут, последующий период реперфузии составил 2 часа. Части животных внутривенно было введено 10 мг/кг Prx VI за 15 минут до ишемии. Для проведения гистологического анализа отбирались образцы тонкой кишки. Было показано, что ишемия/ реперфузия сопровождается разрушением целостности кишечных ворсинок и крипт, инфильтрацией лимфоцитов и окислительным стрессом с серьезным поражением слизистой оболочки. Предварительное введение крысам Prx VI защищает кишечник от И/Р-повреждения, уменьшая последствия окислительного стресса и сохраняя композицию слизистой тонкой кишки. Эти результаты показали, что Prx VI оказывает благоприятное антиоксидантное действие, к тому же ослабляет последствия И/Р-поражения в ущемленном сегменте кишечника.

 

The intestine is an extremely sensitive organ with regard to ischemia/reperfusion damage (I/R) because of its high oxygen reguirement. The aim of this study is to investigate the effects of Peroxiredoxin VI (Prx VI) on preservation of the small intestine after the intestinal ischemia/reperfusion injury in strangulation ileum model. Intestinal ischemia/ reperfusion injury in strangulation model was produced by occlusion of the distal ileum loop and mesentery with its blood vessels for 60 min followed by a 120-min reperfusion period. A group of animals received intravenous injections of 10 mg/kg Prx VI 15 min prior to the intestinal ischemia/reperfusion in the strangulation model. After surgery, part of the intestine was collected for histological analysis. In I/R group a breakdown in the integrity of villi and crypts was revealed, as well as infiltration of lymphocytes, oxidative damage with serious mucosal loss. Prx VI pretreatment to rats with ischemia/reperfusion injury protected the intestine from ischemia/reperfusion injury by reducing oxidative damage and preserving intestinal mucosal composition. These results demonstrated that Prx VI possessed advantageous antioxidant effects as well as effectively attenuated ischemia-reperfusion trauma of the strangulated intestine segment.

 

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The authors declare that there are no conflicts of interest present.