Optimization of the technique for manufacturing the osteoplastic material based on type 1 human collagen and allogeneic bone chips
https://doi.org/10.23873/2074-0506-2023-15-2-177-187
Abstract
Introduction. Biological grafts, including bone chips and collagen, are supposed to be promising in the treatment of bone tissue treatment. Nevertheless, manufacturing of these grafts still needs to be standardized.
Aim. To optimize methodology of osteoplastic material production, based on allogenic 1 type collagen and bone chips.
Material and methods. Osteoplastic material grafts were produced, using with allogeneic bone chips 180-800 µm and type 1 collagen solution in acidic acid. We studied total integrity of graft, collagen quality, morphofunctional properties of line M-22 human cells interacting with different type of osteoplastic material grafts.
Results. Procedures for manufacturing the osteoplastic material did not significantly affect the quality of collagen in its composition, while lyophilized grafts had pronounced acidogenic and toxic effects in cell culture. Soaking osteoplastic material in isotonic solution for 30 min or longer increased its biocompatibility in vitro. Adhesion properties of osteoplastic material widely varied depending on collagen concentration and bone chips size.
Conclusion. Osteoplastic material prepared with allogeneic bone chips of 180–800 µm and collagen solution in acidic acid had pronounced acidogenic and toxic effects in vitro that could be considerably reduced by soaking in isotonic solution. Varying the collagen concentration and the size of bone chips one could produce biocompatible osteoplastic material grafts with high and low adhesion properties.
About the Authors
A. A. Ofitserov
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Andrey A. Ofitserov, Researcher of the Scientific Department of Biotechnologies and Transfusiology,
25%, study design development, obtaining data for analysis, analysis of the obtained data, writing the text of the manuscript
3 Bolshaya Sukharevskaya Sq., Moscow 129090
M. S. Makarov
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Maksim S. Makarov, Сand. Sci. (Biol.), Senior Researcher of the Scientific Department of Biotechnologies and Transfusiology,
25%, study design development, obtaining data for analysis, analysis of the obtained data; writing the text of the manuscript, review of publications on the topic of the article
3 Bolshaya Sukharevskaya Sq., Moscow 129090
M. V. Storozheva
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Mayya V. Storozheva, Researcher of the Scientific Department of Biotechnologies and Transfusiology,
15%, obtaining data for analysis, analysis of the obtained data
3 Bolshaya Sukharevskaya Sq., Moscow 129090
N. V. Borovkova
N.V. Sklifosovsky Research Institute for Emergency Medicine;
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Natalya V. Borovkova, Dr. Sci. (Med.), Head of the Scientific Department of Biotechnologies and Transfusiology; Associate Professor of the Department of Transplantology and Artificial Organs,
25%, study design development, analysis of the obtained data, writing the text of the manuscript
3 Bolshaya Sukharevskaya Sq., Moscow 129090;
1 Ostrovityanov St., Moscow 117997
I. N. Ponomarev
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Ivan N. Ponomarev, Cand. Sci. (Med.), Senior Researcher of the Scientific Department of Biotechnologies and Transfusiology,
10%, obtaining data for analysis, analysis of the obtained data
3 Bolshaya Sukharevskaya Sq., Moscow 129090
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Review
For citations:
Ofitserov A.A., Makarov M.S., Storozheva M.V., Borovkova N.V., Ponomarev I.N. Optimization of the technique for manufacturing the osteoplastic material based on type 1 human collagen and allogeneic bone chips. Transplantologiya. The Russian Journal of Transplantation. 2023;15(2):177-187. https://doi.org/10.23873/2074-0506-2023-15-2-177-187
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