The effect of vibroacoustic therapy on the structural integrity of erythrocytes and platelets in patients with acute exogenous poisoning

Background. Vibroacoustic therapy is used as a non-drug method of correcting respiratory disorders in various diseases, including acute poisoning. The effect of vibroacoustic therapy on blood cells currently remains unexplored.

Objective. To evaluate the impact of vibroacoustic therapy on the morphofunctional rate of erythrocytes and platelets in patients with acute poisoning in patients with acute poisoning.

Material and methods. Thirty eight patients with complicated acute poisoning transferred to the Toxicology Department of the N.V. Sklifosovsky Research Institute for Emergency Medicine were examined. In 19 patients, along with the standard therapy, a vibroacoustic therapy was performed using a VibroLUNG device (the main study group); the other 19 patients received only standard therapy (the comparison group). Vibroacoustic therapy began on the 2nd day after the patient admission to the Toxicology Department. The course of vibroacoustic therapy consisted of 2–9 procedures. Morphofunctional analysis of erythrocytes and platelets in all cases was performed before the start of the vibroacoustic therapy (before treatment) and before discharge from the hospital (1–2 days after completing the vibroacoustic therapy course).

Results. In both groups, before treatment, we noted normal morphofunctional rate of erythrocytes and normal level of platelets with granules in the circulating blood. The level of severely damaged or altered platelets was only slightly higher than normal and did not differ between the groups. Meanwhile, in 50% of patients, platelets had a tendency to spontaneous activation and hyperactivation. Before discharge, the morph functional parameters of erythrocytes and platelets did not significantly change in both groups, while treatment with vibroacoustic therapy allowed to eliminate or reduce spontaneous platelet activation in 7 of 9 patients in the main study group. In the comparison group spontaneous platelet activation was absent in only 3 of 10 patients. In parallel, the time till pneumonia resolution turned statistically significantly reduced, by 1.5 times.

Conclusions. In acute exogenous poisoning with complicated course, the risk of spontaneous platelet activation and hyperactivation increases. Vibroacoustic therapy does not violate the structural integrity of circulating erythrocytes and platelets. Vibroacoustic therapy, using the VibroLUNG device, reduces the tendency of platelets to spontaneous activation and hyperactivation, reduces the time for resolving pneumonia.

1. Ilyashenko KK, Luzhnikov EA. Toxic damage to the respiratory system in acute poisoning. Moscow: MEDICAL Practice Publishing House; 2004. p. 175. (In Russ.).

2. Eremenko AA, Zyulya eva TP, Kalinina AА, Rozina NA. Evaluation of effectiveness of vibroacoustic lung massage in self-breathing patients after cardiosurgical operations. Clinical and Experimental Surgery. Petrovsky Journal. 2020;8(4):126–134. (In Russ.). https://doi.org/10.33029/2308-1198-2020-8-4-126-134

3. Garus YaN, Antoshkieva RM. The effectiveness of treatment of chronic generalized catarrhal gingivitis with mild antiseptic octenisept, vibroacoustic and antioxidant therapy. Astrakhanskiy meditsinskiy zhurnal. 2011;6(4):119–121. (In Russ.).

4. Kikuchi Y, Koyama T. Red blood cell deformability and protein adsorption on red blood cell surface. Am J Physiol. 1984;247(5):H739–H747. PMID: 6496755 https://doi.org/10.1152/ajpheart.1984.247.5.H739

5. Khvatov VB, Makarov MS, Borovkova NV. The morphologic evaluation of adhesive activity of human thrombocytes using vital dye. Klinicheskaya Laboratornaya Diagnostika. 2013;(7):58-61. (In Russ.).

6. Alentiev AY, Belov NA, Nikiforov RY, Polunin EV, Borovkova NV, Evseev AK, et al. Gas permeation and hemocompatibility of novel perfluorinated polymers for blood oxygenation. Membranes and Membrane Technologies. 2018;8(4):217– 223. (In Russ.). https://doi.org/10.1134/S2218117218040028

7. Makarov MS. Morphofunctional properties of spindle-shaped platelets. Bulletin of Experimental Biology and Medicine. 2023;174(5):681–684. (In Russ.). https://doi.org/10.1007/s10517-023-05769-3

8. Popugaev KA, Bakharev SA, Kiselev KV, Samoylov AS, Kruglykov NM, Abudeev SA, et al. Clinical and pathophysiologic aspects of ECMOassociated hemorrhagic complications. PLoS One. 2020;15(10):e0240117. PMID: 33048966 https://doi.org/10.1371/journal.pone.0240117 eCollection 2020.

9. Surovikina MS, Samoilenko VV, Vlasova EA, Vasilenko IA, Suslov VP. The change of plasma and platelet hemostasis in patients with chronic renal failure during hemodialysis. Urology. 2012;(1):25–29. (In Russ.).

10. Yagoda AV, Koroy PV. Pathology of liver and platelet function. Stavropol; 2008. (In Russ.).

11. Shyu KG, Chang CC, Yeh YC, Sheu JR, Chou DS. Mechanisms of ascorbyl radical formation in human platelet-rich plasma. Biomed Res Int. 2014;2014:614506. PMID: 24696859 https://doi.org/10.1155/2014/614506

12. Ma C, Yao Y, Yue QX, Zhou XW, Yang PY, Wu WY, et al. Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B. PLoS One. 2011;6(2):e14692. PMID: 21379382 https://doi.org/10.1371/journal.pone.0014692

13. Lam WA, Chaudhuri O, Crow A, Webster KD, Li TD, Kita A, et al. Mechanics and contraction dynamics of single platelets and implications for clot stiffening. Nat Mater. 2011;10(1):61–66. PMID: 21131961 https://doi.org/10.1038/nmat2903

14. Momot AP, Barkagan ZS. Issledovanie sistemy gemostaza u lits pozhilogo vozrasta: osnovnye tseli i metody. Klinicheskaja gerontologia. 2007;13(4):44-49. (In Russ.).

15. Lindkvist M, Fernberg U, Ljungberg LU, Fälker K, Fernström M, Hurtig-Wennlöf A, et al. Individual variations in platelet reactivity towards ADP, epinephrine, collagen and nitric oxide, and the association to arterial function in young, healthy adults. Thromb Res. 2019;174:5–12. PMID: 30543988 https://doi.org/10.1016/j.thromres.2018.12.008

16. Rakhmatullina DM. The methods of spontaneous platelet aggregation. The Bulletin of Contemporary Clinical Medicine. 2017;10(3):60–65. (In Russ.). https://doi.org/10.20969/VSKM.2017.10(3).60-65

17. Kehrel B, Brodde M. State of the art in platelet function testing. Transfus Med Hemother. 2013;40(2):73– 86. PMID: 23653569 https://doi.org/10.1159/000350469

18. Ermakov AI, Vinogradova TN, Gaikovaya LB, Sirotkina OV, Vavilova TV. Assessment of platelet activation in patients with HIV infection by flow cytometry. Preventive and clinical medicine. 2022;1(82):94–99. (In Russ.). https://doi.org/10.47843/2074-9120_2022_1_94

19. Ponomaryova AD, Leyderman IN, Kasherininov IYu. Acute mesenteric ischemia in critically ill patients. Possibilities of laboratory diagnostics. Systematic literature review and meta-analysis. Russian Sklifosovsky Journal "Emergency Medical Care". 2022;11(2):317–323. (In Russ.). https://doi.org/10.23934/2223-9022-2022-11-2-317-323