The use of autolоgous leucocyte labelling to search for the focus of inflammation in patient with a transplanted kidney
E. V. Migunova,
A. G. Balkarov,
N. V. Shmarina,
K. E. Lazareva,
V. P. Nikulina,
A. Rustambek Uulu,
D. V. Lonshakov,
L. T. Khamidova,
I. V. Dmitriev https://doi.org/10.23873/2074-0506-2025-17-4-452-460
Abstract
Background. Infectious complications in kidney recipients contribute to a significant decrease in the survival rates of transplants and recipients. If it is difficult to determine the source and localization of inflammation in immunocompromised patients using standard research methods, it is possible to use a radionuclide method using labeled autoleukocytes.
Objective. To demonstrate the possibilities of using an autoleukocyte tag in a kidney recipient to search for a focus of inflammatory infiltration.
Material and methods. A 33-year-old patient underwent primary kidney transplantation from a postmortem donor. The early postoperative period was complicated by the development of an infection site, the localization of which could not be identified using standard diagnostic methods. The search for the inflammatory focus was carried out using the radionuclide label of autoleukocytes.
Results. The study of radionuclide labelling of autologous leukocyte made it possible to identify the focus of inflammation, which was represented by infiltrate in the projection of the lower third of the sigmoid colon due to its microperforation. The treatment performed made it possible to cure this infectious complication.
Conclusions. The use of clarifying and non-routine diagnostic methods helps to promptly and accurately determine the location and size of the leukocyte infiltration.
About the Authors
E. V. Migunova
N.V. Sklifosovsky Research Institute for Emergency Medicine; Russian Medical Academy of Continuous Professional Education
Russian Federation
Russian Federation
Ekaterina V. Migunova - Dr. Sci. (Med.), Leading Researcher, Diagnostic Radiology Department, N.V. Sklifosovsky Research Institute for Emergency Medicine; Professor of the Department of Radiology, Radiotherapy, Radiation Hygiene and Radiation Safety named after Academicians A.S. Pavlov and F.G. Krotkov, Russian Medical Academy of Continuous Professional Education.
3 Bolshaya Sukharevskaya Sq., Moscow 129090; 2/1 Bldg. 1 Barrikadnaya St., Moscow 125993
A. G. Balkarov
N.V. Sklifosovsky Research Institute for Emergency Medicine; N.I. Pirogov Russian National Research Medical University (Pirogov University); Research Institute for Healthcare Organization and Medical Management
Russian Federation
Russian Federation
Aslan G. Balkarov - Cand. Sci. (Med.), Head of the Scientific Department of Kidney and Pancreas Transplantation, N.V. Sklifosovsky Research Institute for Emergency Medicine; Associate Professor of the V.P. Demikhov Department of Transplantology and Artificial Organs, N.I. Pirogov Russian National Research Medical University (Pirogov University); Head of the Organizational and Methodological Department for Transplantology, Research Institute for Healthcare Organization and Medical Management.
3 Bolshaya Sukharevskaya Sq., Moscow 129090; 1 Ostrovityanov St., Moscow 117997; 30 Bolshaya Tatarskaya St., Moscow 115184
N. V. Shmarina
N.V. Sklifosovsky Research Institute for Emergency Medicine; N.I. Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
Nonna V. Shmarina - Cand. Sci. (Med.), Senior Researcher, Department of Kidney and Pancreas Transplantation, N.V. Sklifosovsky Research Institute for Emergency Medicine; Associate Professor of the V.P. Demikhov Department of Transplantation and Artificial Organs, N.I. Pirogov Russian National Research Medical University (Pirogov University).
3 Bolshaya Sukharevskaya Sq., Moscow 129090; 1 Ostrovityanov St., Moscow 117997
K. E. Lazareva
N.V. Sklifosovsky Research Institute for Emergency Medicine; Russian University of Medicine
Russian Federation
Russian Federation
Kseniya E. Lazareva - Cand. Sci. (Med.), Endocrinologist, Research Associate, Department of Kidney and Pancreas Transplantation, N.V. Sklifosovsky Research Institute for Emergency Medicine; Associate Professor of the Department of Transplantology and Artificial Organs of the Scientific and Educational Institute "N.A. Semashko Higher School of Clinical Medicine", Russian University of Medicine.
3 Bolshaya Sukharevskaya Sq., Moscow 129090; 4 Dolgorukovskaya St., Moscow 127006
V. P. Nikulina
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Valentina P. Nikulina - Cand. Sci. (Med.), Senior Researcher, Clinical Immunology Laboratory, N.V. Sklifosovsky Research Institute for Emergency Medicine.
3 Bolshaya Sukharevskaya Sq., Moscow 129090
A. Rustambek Uulu
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Alisher Rustambek Uulu - Surgeon, Kidney and Pancreas Transplantation Department, N.V. Sklifosovsky Research Institute for Emergency Medicine.
3 Bolshaya Sukharevskaya Sq., Moscow 129090
D. V. Lonshakov
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Denis V. Lonshakov - Surgeon, Kidney and Pancreas Transplantation Department, N.V. Sklifosovsky Research Institute for Emergency Medicine.
3 Bolshaya Sukharevskaya Sq., Moscow 129090
L. T. Khamidova
N.V. Sklifosovsky Research Institute for Emergency Medicine
Russian Federation
Russian Federation
Layla T. Khamidova - Dr. Sci. (Med.), Head of the Radiology Department, N.V. Sklifosovsky Research Institute for Emergency Medicine.
3 Bolshaya Sukharevskaya Sq., Moscow 129090
I. V. Dmitriev
N.V. Sklifosovsky Research Institute for Emergency Medicine; N.I. Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
Ilya V. Dmitriyev - Dr. Sci. (Med.), Head of the Department of Kidney and Pancreas Transplantation, N.V. Sklifosovsky Research Institute for Emergency Medicine; Associate Professor of the V.P. Demikhov Department of Transplantation and Artificial Organs, N.I. Pirogov Russian National Research Medical University (Pirogov University).
3 Bolshaya Sukharevskaya Sq., Moscow 129090; 1 Ostrovityanov St., Moscow 117997
References
1. Banach DB, Seville MTA, Kusne S. Infection prevention and control issues after solid organ transplantation. In: Ljungman P, Snydman D, Boeckh M. (eds.) Transplant infections. 4th ed. Springer International Publishing Switzerland; 2016. p. 843–867.
2. Huh K, Jeong SJ, Kim YJ, Kang J-M, Kim JM, Park WB, et al. Nationwide survey of infection prevention protocols in solid organ transplantation in South Korea. Korean J Transplant. 2022;36(3):212–220. PMID: 36275990 https://doi.org/10.4285/kjt.22.0036
3. Yu SJ, Heo JH, Choi EJ, Kim JH, Lee HS, Kim SY, et al. Role of multidetector computed tomography in patients with acute infectious colitis. World J Clin Cases. 2022;10(12):3686–3697. PMID: 35647171 https://doi.org/10.12998/wjcc.v10.i12.3686
4. Bafi AT, Tomotani DYPV, de Freitas FGR. Sepsis in solid-organ transplant patients. /hock. 2017;47(1S Suppl 1):12–16. PMID: 27454377 https://doi.org/10.1097/SHK.0000000000000700
5. Carracedo J, Alique M, Vida C, Bodega G, Ceprián N, Morales E, et al. Mechanisms of cardiovascular disorders in patients with chronic kidney disease: a process related to accelerated senescence. Front Cell Dev Biol. 2020;8:185. PMID: 32266265 https://doi.org/10.3389/fcell.2020.00185
6. Kooman JP, Dekker MJ, Usvyat LA, Kotanko P, van der Sande FM, Schalkwijk CG, et al. Inflammation and premature aging in advanced chronic kidney disease. Am J Physiol Renal Physiol. 2017;313(4):F938–F950. PMID: 28701312 https://doi.org/10.1152/ajprenal.00256.2017
7. Ebert T, Pawelzik SC, Witasp A, Arefin S, Hobson S, Kublickiene K, et al. Inflammation and premature ageing in chronic kidney disease. Toxins (Basel). 2020;12(4):227. PMID: 32260373 https://doi.org/10.3390/toxins12040227
8. Franceschi C, Bonafè M, Valensin S, Olivieri F, De Luca M, Ottaviani E, et al. Inflamm-aging. An evolutionary perspective on immunosenescence. Ann N Y Acad Sci. 2000;908:244–254. PMID: 10911963 https://doi.org/10.1111/j.1749-6632.2000.tb06651.x
9. De La Fuente M, Miquel J. An update of the oxidation-inflammation theory of aging: the involvement of the immune system in oxi-inflamm-aging. Curr Pharm Des. 2009;15(263):3003–3026. PMID: 19754376 https://doi.org/10.2174/138161209789058110
10. Syed-Ahmed M, Narayanan M. Immune dysfunction and risk of infection in chronic kidney disease. Adv Chronic Kidney Dis. 2019;26(1):8–15. PMID: 30876622 https://doi.org/10.1053/j.ackd.2019.01.004
11. Kato S, Chmielewski M, Honda H, Pecoits-Filho R, Matsuo S, Yuzawa Y, et al. Aspects of immune dysfunction in end-stage renal disease. Clin J Am Soc Nephrol. 2008;3(5):1526–1533. PMID: 18701615 https://doi.org/10.2215/CJN.00950208
12. Kurts C, Panzer U, Anders HJ, Rees AJ. The immune system and kidney disease: basic concepts and clinical implications. Nat Rev Immunol. 2013;13(10):738–753. PMID: 24037418 https://doi.org/10.1038/nri3523
Review
For citations:
Migunova E.V., Balkarov A.G., Shmarina N.V., Lazareva K.E., Nikulina V.P., Rustambek Uulu A., Lonshakov D.V., Khamidova L.T., Dmitriev I.V. The use of autolоgous leucocyte labelling to search for the focus of inflammation in patient with a transplanted kidney. Transplantologiya. The Russian Journal of Transplantation. 2025;17(4):452-460. (In Russ.) https://doi.org/10.23873/2074-0506-2025-17-4-452-460
Views:
30
Email this article 