The effects of Peroxiredoxin VI on the preservation of the small intestine in rats after ischemia/reperfusion damage
Abstract
The intestine is an extremely sensitive organ with regard to ischemia/reperfusion damage (I/R) because of its high oxygen reguirement. The aim of this study is to investigate the effects of Peroxiredoxin VI (Prx VI) on preservation of the small intestine after the intestinal ischemia/reperfusion injury in strangulation ileum model. Intestinal ischemia/ reperfusion injury in strangulation model was produced by occlusion of the distal ileum loop and mesentery with its blood vessels for 60 min followed by a 120-min reperfusion period. A group of animals received intravenous injections of 10 mg/kg Prx VI 15 min prior to the intestinal ischemia/reperfusion in the strangulation model. After surgery, part of the intestine was collected for histological analysis. In I/R group a breakdown in the integrity of villi and crypts was revealed, as well as infiltration of lymphocytes, oxidative damage with serious mucosal loss. Prx VI pretreatment to rats with ischemia/reperfusion injury protected the intestine from ischemia/reperfusion injury by reducing oxidative damage and preserving intestinal mucosal composition. These results demonstrated that Prx VI possessed advantageous antioxidant effects as well as effectively attenuated ischemia-reperfusion trauma of the strangulated intestine segment.
About the Authors
A. E. Gordeeva
Institute of сell biophysics Russian Academy of Sciences, Pushchino
Russian Federation
Russian Federation
V. G. Sharapov
Institute of сell biophysics Russian Academy of Sciences, Pushchino
Russian Federation
Russian Federation
V. I. Novoselov
Institute of сell biophysics Russian Academy of Sciences, Pushchino
Russian Federation
Russian Federation
E. E. Fesenko
Institute of сell biophysics Russian Academy of Sciences, Pushchino
Russian Federation
Russian Federation
A. A. Temnov
N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow
Russian Federation
Russian Federation
M. Sh. Khubutiya
N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow
Russian Federation
Russian Federation
References
1. Mallick I.H., Yang W., Winslet M.C., Seifalian A.M.. Ischemia-reperfusion injury of the intestine and protective strategies against injury. Dig. Dis. Sci. 2004; 49 (9): 1359–1377.
2. Laws E.G., Freeman D.E. Significance of reperfusion injury after venous strangulation obstruction of equine jejunum. J. Invest. Surg. 1995; 8 (4): 263–270.
3. Akcakaya A., Alimoglu O., Sahin M., Abbasoglu S.D. Ischemia-reperfusion injury following superior mesenteric artery occlusion and strangulation obstruction. J. Surg. Res. 2002; 108 (1): 39–43.
4. Bagnenko S.F., Sinenchenko G.I., Chupris V.G. Laparoskopicheskaya diagnostika i lechenie ostroy spaechnoy tonkokishechnoy neprokhodimosti [Laparoscopic diagnosis and treatment of acute adhesive small bowel obstruction]. Vestnik khirurgii im. I.I. Grekova. 2009; 1: 27–30. (In Russian).
5. Grinev M.V., Bromber A.V. Ishemiyareperfuziya – universal'nyy mekhanizm patogeneza kriticheskikh sostoyaniy neotlozhnoy khirurgii [Ischemia-reperfusion universal mechanism of pathogenesis of critical states of emergency surgery]. Vestnik khirurgii im. I.I. Grekova. 2012; 4: 94–100. (In Russian).
6. Bilenko M.V. Ishemichekie i reperfuzionne povrezhdeniya organov (molekulyarnye mekhanizmy, puti preduprezhdeniya i lecheniya [Ishemichekie and reperfusion injury of organs (molecular mechanisms ways to prevent and treat]. Moscow: Meditsina Publ., 1989. 368 p. (In Russian).
7. Kapel'ko V.I. Evolyutsiya, kontseptsiya i metabolicheskaya osnova ishemicheskoy disfunktsii miokarda [The evolution of the concept and the metabolic basis of ischemic myocardial dysfunction]. Kardiologiya. 2005; 9: 55–61. (In Russian).
8. Harrison R. Structure and function of xanthine oxidoreductase: Where are we now? Free Radic. Biol. Med. 2002; 33 (6): 774–797.
9. Peshenko I.V., Novoselov V.I., Evdokimov V.A., [et al.]. Identification of a 28 kDa secretory protein from rat olfactory epithelium as a thiol-specific antioxidant. Free Radic. Biol. Med. 1998; 25 (6): 654–659.
10. Novoselov V.I., Mubarakshina E.K., Yanin V.A., [et al.]. Rol' antioksidantnykh sistem v regeneratsii epiteliya trakhei posle termicheskogo ozhoga verkhnikh dykhatel'nykh putey [The role of antioxidant systems in the regeneration of the epithelium of the trachea after thermal burns of the upper respiratory tract]. Pul'monologiya. 2008; 6: 80–83. (In Russian).
11. Novoselov V.I. Rol' peroksiredoksinov pri okislitel'nom stresse v organakh dykhaniya [The role of peroxiredoxin in oxidative stress in the respiratory tract]. Pul'monologiya. 2012; 1: 83–87. (In Russian).
12. Volkova A.G., Sharapov M.G., Ravvin V.K., [et al.]. Effekt razlichnykh fermentov-antioksidantov na regenerativnye protsessy v epitelii trakhei posle khimicheskogo ozhoga [The effect of different antioxidant enzymes on regenerative processes in the epithelium of the trachea after chemical burn]. Pul'monologiya. 2014; 2: 84–90. (In Russian).
13. Novoselov V.I., Ravin V.K., Sharapov M.G., [et al.]. Modifitsirovannye perokiredoksiny kak prototipy lekarstvennykh preparatov moshchnogo antioksidantnogo deystviya [Perokiredoksiny modified as prototypes of drugs powerful antioxidant action]. Biofizika. 2011; Suppl. 56 (5): 873–880. (In Russian)
14. Novoselov V.I., Baryshnikova L.M., Yanin V.A., [et al.]. Vliyanie peroksiredoksina VI na zazhivlenie rezanoy rany [Effect of peroxiredoxin VI in healing incised wounds]. Dokl. Akademii Nauk RF. 2003; Suppl. 393 (3): 412–414. (In Russian).
15. Chiu C.J., McArdle A.H., Brown R., [et al.]. Intestinal mucosal lesion in lowflow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch. Surg. 1970; 101 (4): 478–483.
16. Halliwell B., Gutteridge J. Free Radicals in Biology and Medicine. 4rd ed. New York: Oxford University Press, 2007. 704.
17. Bagnenko S.F., Sinenchenko G.I., Kurygin A.A., Chupris V.G. Korrektsiya reperfuzionnoy disfunktsii pri ostroy kishechnoy neprokhodimosti [Correction reperfusion dysfunction in acute intestinal obstruction]. Vestnik khirurgii im. I.I. Grekova. 2008; 4: 32–35. (In Russian).
18. Novoselov S.V., Peshenko I.V., Popov V.V., [et al.]. Localization of the 28-kDa peroxiredoxin in rat epithelial tissues and its antioxidant properties. Cell Tissue Res. 1999; 298 (3): 471–480.
19. Wang X., Phelan S.A., Forsman Semb K., [et al.]. Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress. J. Biol. Chem. 2003; 278 (27): 25179–25190.
20. Sharapov M.G., Ravin V.K., Novoselov V.I. Peroksiredoksiny – mnogofunktsional'nye fermenty [Peroxiredoxins - multifunctional enzymes]. Molekulyarnaya biologiya. 2014; Suppl. 4 (48): 600–628. (In Russian).
21. Matsuo H., Hirose H., Sakamoto K., Yasumura M. Experimental studies to estimate the intestinal viability in a rat strangulated ileus model using a dielectric parameter. Dig Dis Sci. 2004; 49 (4): 633–63.
22. Basarab D.A., Bagdasarov V.V., Bagdasarova E.A., [et al.]. Patofiziologicheskie aspekty problemy ostroy intestinal'noy ishemii [Патофизиологические аспекты проблемы острой интестинальной ишемии]. Infektsii v khirurgii. 2012; 2: 6–13. (In Russian).
Review
For citations:
Gordeeva A.E., Sharapov V.G., Novoselov V.I., Fesenko E.E., Temnov A.A., Khubutiya M.Sh. The effects of Peroxiredoxin VI on the preservation of the small intestine in rats after ischemia/reperfusion damage. Transplantologiya. The Russian Journal of Transplantation. 2014;(4):21-27.
Views:
650
Email this article 