Understanding of the nitric oxide (NO) role in the pathogenesis of acute and chronic disorders of the pulmonary circulation contributed to the introduction of inhaled NO (iNO) in the complex therapy of patients after lung transplantation. Inhaled NO can form methemoglobin (MetHb) and nitrate. The aim of the study was to investigate the relationship between iNO and nitrite/nitrate (NOx) and MetHb levels in the blood serum of 7 patients after lung transplantation. Duration of iNO therapy was 3±1 days, the data were reviewed at 1 to 10 days after  transplantation. Patients showed significant improvement of the NOx level from day 1 to 5 after surgery, meanwhile the MetHb concentration remained within the normal range. The significant positive correlations were found between iNO and NOx concentrations, between iNO and MetHb concentrations, and between NOx and MetHb concentrations. The obtained results suggest that when using iNO in patients in lung transplantation, the concentration of the administered iNO, as well as blood levels of MetHb and NOx must be closely monitored.

Purpose: This study focuses on the development of the method of introduction of magnetic microparticles in the cytoplasm of HEK-293 cell line with their subsequent fixation under the retina of the eye.

Materials and Methods. Magnetic particles (d = 2,8 mm) were treated with pluronic and injected into the cytoplasm of HEK-293 cell line, expressing GFP. The surgery was made under general anesthesia. HEK-293 containing magnetic particles were injected into the subretinal space of rabbit eyes (eyes 96, 48 rabbits) using original dosing device. In the experimental group (48 eyes, 24 rabbits) we fixed episcleral magnetic implant to hold cells in local place. In the control group (48 eyes, 24 rabbit) magnetic implant was not fixed. After the surgery all animals were examined using biomicroscopy, ophthalmoscopy with photographic recording, ultrasound, computed tomography and morphological study in certain terms (1, 3, 5, 7, 14, 21 day and 1 month).

Results: The introduction of the magnetic particles into the cytoplasm of HEK 293 cell line has no effect on cell viability. HEK-293 containing magnetic particles remains in the place of injection during 21 days in rabbit eyes, where the magnetic implants were fixed (in control group during 3 days).

Conclusions: Using of cells containing magnetic particles with fixation of the magnetic implant can be a promising method for cell therapy for the treatment of retinal diseases.

The intestine is an extremely sensitive organ with regard to ischemia/reperfusion damage (I/R) because of its high oxygen reguirement. The aim of this study is to investigate the effects of Peroxiredoxin VI (Prx VI) on preservation of the small intestine after the intestinal ischemia/reperfusion injury in strangulation ileum model. Intestinal ischemia/ reperfusion injury in strangulation model was produced by occlusion of the distal ileum loop and mesentery with its blood vessels for 60 min followed by a 120-min reperfusion period. A group of animals received intravenous injections of 10 mg/kg Prx VI 15 min prior to the intestinal ischemia/reperfusion in the strangulation model. After surgery, part of the intestine was collected for histological analysis. In I/R group a breakdown in the integrity of villi and crypts was revealed, as well as infiltration of lymphocytes, oxidative damage with serious mucosal loss. Prx VI pretreatment to rats with ischemia/reperfusion injury protected the intestine from ischemia/reperfusion injury by reducing oxidative damage and preserving intestinal mucosal composition. These results demonstrated that Prx VI possessed advantageous antioxidant effects as well as effectively attenuated ischemia-reperfusion trauma of the strangulated intestine segment.